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Vitamin D Testing Market Size, Share & Industry Analysis, By Product (25-Hydroxy Vitamin D Testing, 1,25-Dihydroxy Vitamin D Testing, and 24,25-Dihydroxy Vitamin D Testing), By Application (Clinical Testing and Research Testing), By End-User (Standalone Clinical Laboratories, Hospitals & Clinics, Others) and Regional Forecast, 2024-2032

Last Updated: July 01, 2024 | Format: PDF | Report ID: FBI100968



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The global vitamin D testing market size was valued at USD 652.2 million in 2023. The market is projected to grow from USD 682.2 million in 2024 to USD 1,021.3 million by 2032, exhibiting a CAGR of 5.2% during the forecast period.

Increasing sedentary lifestyles and less exposure to sunlight are resulting in the rising prevalence of vitamin D deficiency in developed and emerging countries. For instance, according to an article published by BioMed Central Ltd., the prevalence of vitamin D deficiency among the Indian population was almost 67.0% in 2021.

This, along with the clinically established connection between several serious and chronic diseases with deficiency of vitamin D, is increasing the demand and adoption of the products used in vitamin D tests during the study period. Additionally, rising initiatives for the awareness regarding the level of vitamins, such as vitamin D, in both developing and developed nations and the emergence of new research demonstrating the importance of vitamin D in an individual’s overall health are propelling the vitamin D testing market growth. This factor, coupled with increasing investments by major players in research and development for new testing technologies, is also boosting the market's growth globally.

The outbreak of COVID-19 had a negative impact on the market. Several key market players, including DiaSorin S.p.A., Abbott, and others, witnessed a significant decline in the revenue from their vitamin testing products. For instance, DiaSorin S.p.A. experienced a decline of 25.9% in the sales of products used in vitamin D tests in 2020 as compared to the previous fiscal year.

Additionally, stringent lockdown and COVID-19 restrictions resulted in a significant decrease in routine blood tests, including vitamin D tests and lipid profile. This also resulted in negative market growth during the pandemic.

However, according to a number of KOL interviews, it was reported that there had been a tremendous rise in patient volume during 2021. Also, increased health awareness of the post-COVID-19 situation and the introduction of advanced point of care testing kits are fostering the adoption rate of devices during the study period. Also, the key players operating in the market experienced a significant increase in revenue and returned to their pre-pandemic level in 2021.

Vitamin D Testing Market Trends

Emphasis Toward Quantitative Determination of 24,25-dihydroxy Vitamin D for Significant Evaluation of Vitamin D

24,25-dihydroxy vitamin D is a major form of vitamin D that is closely linked with the 1,25-dihydroxyvitamin D. Additionally, the ratio of 25-hydroxy vitamin D and 24,25-dihydroxy vitamin D has the clinical significance of determining the level of vitamin D in the human body. Also, according to a number of research publications and KOL interviews, it was reported that 24,25-dihydroxy vitamin D is effective in fracture healing. As a result, recent and ongoing advances in R&D are focusing on determining the level of 24,25-dihydroxy vitamin D.

Along with this, a recent trend was observed for adopting the point-of-care testing kits in place of lab-based tests. According to an article published by Ipsos in 2022, it was reported that several distinct advantages, including lower turn-around time, ease of use, and fewer requirements of professional guidance, are some of the factors for the preferential shift from lab-based tests to point-of-care tests.

Furthermore, technological advancements led to the development of novel testing methods for Vitamin D, including electrochemical biosensors, liquid-chromatography-mass-spectroscopy, and immunoassays. These advancements enhance accuracy, sensitivity, and specificity when assessing vitamin D levels, leading to improved clinical outcomes.

  • For instance, as per the data published by IntechOpen in May 2023, the electrochemical biosensors hold significant promise for investigating vitamin D metabolism, understanding deficiencies, and exploring their links to various diseases in research studies. Moreover, these tools can be integrated into clinical practice to conduct routine assessments of vitamin D levels, ensuring timely and accurate patient monitoring. 

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Vitamin D Testing Market Growth Factors

Rising Prevalence of Vitamin D Deficiency and Associated Diseases to Lead to Market Growth

The increasing prevalence of vitamin D deficiency and the rising prevalence of associated diseases, including osteoporosis and Inflammatory Bowel Disease (IBD) are some of the major factors resulting in the increasing demand for this procedure.

  • For instance, according to an article published by WebMD LLC in April 2020, it was reported that the global prevalence of IBD is 396 cases per 100,000 persons annually.

Also, this huge patient population suffering from vitamin D deficiency is seeking medical attention for the treatment of vitamin D deficiency and resulting in a rising economic burden. Eventually, this led to increased initiatives for a number of awareness campaigns regarding the importance of vitamin D by the government and other for-profit and non-profit organizations.

  • For instance, according to a report published by The Indian Express Ltd., in 2021, Royal DSM, a nutrition firm, launched a digital-led campaign in India with the goal of raising awareness about the importance of increasing vitamin D levels through good nutrition. This is responsible for the growing adoption of routine vitamin D testing and fueling the market growth during the study period.

Similarly, the demand for vitamin D testing has increased due to various factors, including rising incidences of bone disorders, a growing elderly population, advancements in testing technology, and government initiatives promoting vitamin D testing.

  • For instance, as per the data provided by WHO, in 2019, the global population of individuals aged 60 years and older stood at 1.00 billion. By 2023, this figure is projected to rise to 1.40 billion; by 2050, it is expected to reach 2.10 billion.


Growing Initiatives to Limit the Overuse of Vitamin D Testing to Limit Market Growth

Vitamin D deficiency is one of the most critical factors linked to an individual's overall health. However, a marked increase was observed in repetitive testing in the developed countries, including the U.S., Germany, and others. This is resulting in the overuse of healthcare resources, such as test kits, reagents, and others, and subsequently increases the healthcare burdens over z period of time.

Thus, a number of countries implemented certain regulatory guidelines regarding the vitamin testing frequency and usage of test kits. This significantly decreased the overutilization of healthcare resources as well as expenses and subsequently hindered the market growth during the study period.

  • For instance, according to a study published by the Royal College of General Practitioners in 2021, a decrease of around 23.3% in vitamin D tests was observed after providing a multi-level intervention program based on training, monitoring, and feedback on 23 primary care health centers in the Netherlands.

Additionally, the high cost associated with the test is also hindering the adoption of vitamin D tests among the general population in the emerging countries, including Brazil, South Africa, and others.

  • For instance, according to KINGS COLLEGE HOSPITAL in Dubai, the average procedural cost of a vitamin D test along with consultation is around USD 41.0.

Furthermore, researchers are conducting studies to check the efficiency of predictive models in the assessment of Vitamin D levels. This alternative method in vitamin D testing can reduce the demand for traditional laboratory tests. It may be perceived as more convenient or cost-effective by healthcare providers and patients.

  • For instance, as per the data published by Sec. Bone Research in February 2024 was conducted by utilizing machine learning to predict the risk of vitamin D deficiency by collecting data through straightforward community interviews. The study findings revealed that the XGBoost-based prediction tool operates with exceptional precision, effectively identifying the risk of vitamin D deficiency within the community.

Vitamin D Testing Market Segmentation Analysis

By Product Analysis

25-Hydroxy Vitamin D Test to Hold Dominant Position with Rising Vitamin D Deficiency

Based on product, the market is segmented into 25-hydroxy vitamin D testing, 1,25-dihydroxy vitamin D testing, and 24,25-dihydroxy vitamin D testing.

25-hydroxy vitamin D test dominated the market in 2023 and is anticipated to hold the dominant position during the study period. Determining the level of 25-hydroxy vitamin D is recognized as the gold standard of vitamin D tests. Moreover, the rising prevalence of vitamin D deficiency and insufficiency is surging the demand for this testing around the globe and subsequently supporting the segmental dominance of this segment. Also, diversified application of 25-hydroxy vitamin D for the nutritional assessment has been instrumental in the growth of this segment.

On the other hand, 1,25-dihydroxy vitamin D testing is anticipated to grow with the highest CAGR during the study period. Increasing application of 1,25-dihydroxy vitamin D in the diagnosis of sarcoidosis, and renal dysfunction, in combination with parathyroid hormone, is fueling the adoption rate of this test type. For instance, according to the American Lung Association, in 2020, almost 0.2 million sarcoidosis cases were diagnosed in the U.S. per year.

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By Application Analysis

Increasing Application of Vitamin D Test Boosts Clinical Testing

Based on application, the market is segmented into clinical testing and research testing. The clinical testing segment held the lion’s share in the market in 2023. The increasing prevalence of vitamin D deficiency, along with rising initiatives for the awareness of this testing, is resulting in a higher demand for vitamin D test procedures. Also, the introduction of advanced point-of-care testing kits for the quantitative diagnosis of vitamin D also drives the segmental growth during the study period.

On the other hand, increasing investment by the market players for the introduction of advanced test kits along with the growing utilization of vitamin D tests in a number of healthcare research procedures is anticipated to boost the growth of the research testing segment significantly.

By End-User Analysis

Availability of Advanced Patient Care in Standalone Clinical Laboratories to Propel the Vitamin D Testing Market Outlook

Based on end-user, the market is categorized into standalone clinical laboratories, hospitals & clinics, and others. Vitamin D test is generally prescribed by general practitioners and internal medicine practitioners. However, this test is done at standalone clinical laboratories due to the presence of advanced laboratory equipment, reagents, and others. This eventually fueled the segmental growth during the study period.

Also, increasing investment for the expansion of the standalone clinical laboratories is also boosting the growth opportunity globally. For instance, Mayo Clinic invested USD 49.0 million in 2021 for the laboratory expansion project in Rochester.

The hospitals & clinics segment is also anticipated to grow with a significant CAGR during the study period. Increasing initiatives for the development of hospital infrastructure and a rising number of research surveys done in hospital and clinical settings are anticipated to support the growth of this segment.


Europe Vitamin D Testing Market Size, 2023 (USD Million)

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The market in Europe stood at USD 231.8 million in 2023. This region accounted for the major vitamin D testing market share in the global market. Approval of a large number of point of care testing products by the regulatory authority of this region, along with the increasing prevalence of vitamin D deficiency, is fueling the demand and adoption of the products in this region. For instance, according to an article published by the National Library of Medicine in 2019, it was reported that around 30.0-60.0% of the people in western, southern and eastern Europe were suffering from vitamin D deficiency.

North America accounted for the second position in value in the global market. Increasing prevalence of chronic kidney disease is fueling the demand for diagnosis of 1,25-dihydroxy vitamin D. For instance, according to according to the U.S. Department of Health & Human Services, approximately 15.0% of the U.S. adults were suffering from CKD in 2021. This, in turn, is increasing the adoption rate of vitamin D diagnostics products and subsequently bolstering the market growth in this region.

Asia Pacific is expected to emerge as the fastest-growing region during the forecast period. Developing healthcare infrastructure along with rising penetration of these products in Chinese and Japanese markets is fostering the high growth rate of this region.

Furthermore, Latin America and the Middle East & Africa are likely to witness a significant CAGR during the study period. The presence of a large patient population in these regions and inorganic growth strategies, including mergers and acquisitions between the major players and the regional for-profit and non-profit organizations are anticipated to fuel the market growth in these regions. For instance, in October 2020, BIOMÉRIEUX collaborated with the African Union Member States (AMSP) to supply high-quality diagnostics, consumables, and medical equipment to the African community.

List of Key Companies in Vitamin D Testing Market

Advanced Product Offerings & Geographical Expansion to Help Players Gain Top Position

The market structure is consolidated, with a few players operating in the market. F. Hoffmann-La Roche Ltd, DiaSorin S.p.A., and Abbott account for the lion’s share in the market. The introduction of advanced cutting-edge technology to provide precise results to the patient is one of the major factors for the market dominance of these players. For instance, in January 2022, F. Hoffmann-La Roche Ltd. introduced Cobas Infinity Edge, a cloud-based platform for integrating and managing point-of-care data available for use in clinical settings worldwide.

Other prominent players operating in the market are BIOMÉRIEUX, Beckman Coulter, Inc., Siemens Healthcare GmbH, Quidel Corporation, Thermo Fisher Scientific Inc., RECIPE Chemicals + Instruments GmbH, and Immunodiagnostic Systems Holdings PLC.



  • May 2020: Abbott announced the availability of ID NOW in the U.S., a point-of-care quick test that provides results in minutes rather than taking days or weeks to deliver the test results.

  • March 2020 – Thermo Fisher Scientific Inc. announced the availability of Cascadion SM Clinical Analyzer for Cascadion SM 25-hydroxy vitamin D assay in the U.S. with an aim to expand its brand presence.


An Infographic Representation of Vitamin D Testing Market

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The global market research report provides a detailed analysis and focuses on crucial aspects such as leading companies, applications, disposable types, and distribution channels. Also, the report offers insights into the market, current trends, and highlights the vital industry developments. In addition to the factors mentioned above, the report encompasses various factors that have contributed to the growth of the market in recent years.

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Report Scope & Segmentation



Study Period


Base Year


Estimated Year


Forecast Period


Historical Period


Growth Rate 

CAGR of 5.2% from 2024-2032


Value (USD million)



By Product

  • 25-Hydroxy Vitamin D Testing

  • 1,25-Dihydroxy Vitamin D Testing

  • 24,25-Dihydroxy Vitamin D Testing

By Application

  • Clinical Testing

  • Research Testing

By End-User

  • Standalone Clinical Laboratories

  • Hospitals & Clinics

  • Others

By Geography

  • North America (By Product, By Application, By End-User, and By Country)

    • U.S.

    • Canada

  • Europe (By Product, By Application, By End-User, and By Country/Sub-region)

    • U.K.

    • Germany

    • France

    • Italy

    • Spain

    • Scandinavia

    • Rest of Europe

  • Asia Pacific (By Product, By Application, By End-User, and By Country/Sub-region)

    • Japan

    • China

    • India

    • Australia

    • Southeast Asia

    • Rest of Asia Pacific

  • Latin America (By Product, By Application, By End-User, and By Country/Sub-region)

    • Brazil

    • Mexico

    • Rest of Latin America

  • Middle East & Africa (By Product, By Application, By End-User, and By Country/Sub-region)

    • South Africa

    • GCC Countries

  • Rest of Middle East & Africa

Frequently Asked Questions

Fortune Business Insights says that the global market size was USD 652.2 million in 2023 and is projected to reach USD 1,021.3 million by 2032.

In 2023, the Europe market value stood at USD 231.8 million.

The market will exhibit steady growth at a CAGR of 5.2% during the forecast period (2024-2032).

The 25-hydroxy vitamin D testing segment is expected to be the leading segment by product in this market during the forecast period.

The rising prevalence of vitamin D deficiency along with advanced product launches by key market players will drive the market growth.

F. Hoffmann-La Roche Ltd, DiaSorin S.p.A., and Abbott are some of the leading players in the global market.

Europe dominated the global market in 2023.

The increasing prevalence of vitamin D deficiency and rising initiatives for the awareness regarding vitamin D testing are expected to drive the adoption of the products.

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